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Human FGF-10 Recombinant Protein  Cite:0    分享

货号： PR0003
复制产品信息
规格:
5UG
单位:
支
单价:￥1120.00
产品简介
Storage & Handling
Storage/Stability
Specifications
注意事项
产品简介
  • Source
    E.coli
  • Synonyms
    Fibroblast Growth Factor-10, FGFA, Keratinocyte Growth Factor-2
  • Description
    FGF-10 is a heparin-binding growth factor that belongs to the FGF family. Proteins of this family play a central role during prenataldevelopment, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF10 is most related to KGF/FGF-7, and is expressed during the development and, preferentially, in adult lungs. It signals throughFGFR 2b.--Recombinant Human FGF-10 is a 19.3 kDa protein consisting of 170 amino acid residues
  • Sequence
    MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEIGVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTSAHFLPMVVHS
Storage & Handling
  • Handling
    Centrifuge vial prior to opening. Do not vortex after reconstitution. Avoid repeated freeze-thaw cycles
  • Reconstitution
    Initially reconstitute in 5mM Sodium Phosphate, pH 7.4 to 0.1-1.0 mg/ml. Store at 2°C to 8°C for up to 1week or prepare for extended storage
  • Extended Storage
    After initial reconstitution, further dilute in a buffer containing a carrier protein or stabilizer (e.g. 0.1%BSA). Store working aliquots at -20˚C to -80˚C
Storage/Stability
Product FormTemperature Storage Time
Lyophilized-20˚C to -80˚CDecember 2029
Lyophilized4°C6 months
LyophilizedRoom Temperature1 month 
Reconstituted 2°C to 8°C1 week 
Extended Storage-20˚C to -80˚C 3 months


Specifications
  • Formulation
    Sterile filtered through a 0.2-micron filter. Lyophilized from 5mM Sodium Phosphate pH 7.4 + 80mM NaCl.
  • Authenticity
    Verified by N-terminal and Mass Spectrometry analyses (when applicable)
  • Purity
    ≥ 95% by SDS-PAGE gel and HPLC analyses
  • Endotoxin
    Endotoxin level is < 0.1 ng/μg of protein (< 1 EU/μg)
  • Protein Content
    Verified by UV Spectroscopy and/or SDS-PAGE gel
  • Biological Activity
    Assay #1:-Determined by dose-dependent ability to reduce tetrazolium salt, WST-8, by dehydrogenase activities ofBaF3 cells expressing FGF receptors using Cell Counting Kit-8 (CCK-8). The expected-ED50 is ≤ 0.5 ng/mlcorresponding to a specific activity of ≥ 2 x 106-units/mg.Assay #2:-Determined by a cell proliferation assay using 4MBr-5 cells. The expected-ED50-for this effect is0.5-15.0 ng/ml
注意事项

1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。

2.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。

3.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。 


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