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FGF-2 Protein, Human,Recombinant (Thermostable)  Cite:0    分享

货号： IM38064
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规格:
5μg 10μg 20μg 50μg 100μg 200μg
单位:
支
单价:￥520.00
产品简介
产品简介
运输与保存
注意事项
产品简介
  • 产品名称
    FGF-2 Protein, Human,Recombinant (Thermostable)
  • 别名
    Heparin-binding growth factor 2, HBGF-2, Fibroblast growth factor 2, FGFB, FGF-2, FGF2, bFGF, Basic fibroblast growth factor
  • 种属
    Human
  • 表达系统
    E. coli
  • 标签
    Tag Free
  • 氨基酸序列
    Met1-Ser155
  • 蛋白构建
    Met1-Ser155 with modifications
  • 蛋白纯度
    Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
  • 蛋白性状
    Lyophilized powder
  • 分子量
    17.2 kDa (Predicted); 16 kDa (reducing condition)
  • 缓冲液
    Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 5% sucrose, 0.02% Tween 80, pH 8.0.
  • 复溶方法
    Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
  • 内毒素
    < 0.1 EU/µg as determined by LAL test.
  • 生物活性
    Measured by its ability to induce FGF reporter activity in 293T human embryonic kidney cells.The ED50 for this effect is 0.46 ng/mL.
  • 研究背景
    Fibroblast growth factors (FGF) are a family of heparin-binding secreted proteins that stimulate cell proliferation and differentiation in a wide variety of tissues. FGFs play important roles in diverse biological functions both in vivo and in vitro, including mitogenesis, cellular migration, differentiation, angiogenesis, and wound healing. Human embryonic stem cell (hESC) cultures require FGF basic (also known as FGF-2 or bFGF) in cell culture media to remain in an undifferentiated and pluripotent state. Thermostable FGF basic was engineered for enhanced stability in culture media, without modification of its biological function. FGF basic is a required component of stem cell culture media for maintaining cells in an undifferentiated state. Because FGF basic is unstable, daily media changes are needed. The thermostable FGF basic that supports a 2-day media change schedule, so no media changes are required over a weekend. This thermostable FGF basic was more stable than FGF basic in biochemical studies, and maintained cell growth, pluripotency and differentiation potential with a 2-day feeding schedule.
产品简介

FGF-2 Protein, Human,Recombinant (Thermostable) is expressed in E. coli expression system. The predicted molecular weight is 17.2 kDa and the accession number is P09038-2.

运输与保存
  • Shipping
    In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
  • Stability & Storage
    It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
注意事项

1. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。

2. 为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。

3. 实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。

 

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