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DNA Ligase IV (phospho Thr650) Rabbit Polyclonal Antibody  Cite:0    分享
抗体类满2送1  

货号： IM51788
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  • 种属：H，R
  • 用途：ELISA，IHC-P，IF-P，IF-F，IF-ICC
规格:
50μl 100μl
单位:
支
单价:￥1600.00
产品简介
储存与保存
注意事项
产品简介
  • 产品名称
    DNA Ligase IV (phospho Thr650) Rabbit Polyclonal Antibody
  • 别名
    LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
  • 类别
    抗体产品
  • 基因名称
    LIG4
  • 蛋白名称
    DNA ligase 4
  • Clonality
    Polyclonal
  • 推荐应用
    IHC-P,IF-P,IF-F,IF-ICC,ELISA
  • 稀释度
    IHC-P 1:100-1:300, ELISA 1:10000, IF-P/IF-F/IF-ICC 1:50-200
  • 反应种属
    Human,Rat,Mouse
  • 浓度
    1 mg/ml
  • 存储缓冲液
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
  • Human Gene ID
    3981
  • Human Swissprot No.
    P49917
  • Mouse Swissprot No.
    Q8BTF7
  • 免疫原
    The antiserum was produced against synthesized peptide derived from human DNA Ligase 4 around the phosphorylation site of Thr650. AA range:616-665
  • 特异性
    Phospho-DNA Ligase IV (T650) Polyclonal Antibody detects endogenous levels of DNA Ligase IV protein only when phosphorylated at T650.
  • 参考分子量
    104kDa
  • 宿主
    Rabbit
  • 同种型
    Rabbit,IgG
  • 背景介绍
    The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],
  • 组织表达
    Testis, thymus, prostate and heart.
  • 细胞定位
    Nucleus
  • 信号通路
    Non-homologous end-joining;
  • 功能
    catalytic activity:ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).,cofactor:Magnesium.,disease:Defects in LIG4 are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-negative/NK-cell-positive with sensitivity to ionizing radiation (RSSCID) [MIM:602450]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Individuals affected by RS-SCID show defects in the DNA repair machinery necessary for coding joint formation and the completion of V(D)J recombination. A subset of cells from such patients show increased radiosensitivity.,disease:Defects in LIG4 are the cause of LIG4 syndrome [MIM:606593]. This disease is characterized by immunodeficiency and developmental and growth delay. Patients display unusual facial features, microcephaly, growth and/or developmental delay, pancytopenia, and various skin abnormalities.,function:Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.,online information:DNA ligase entry,online information:LIG4 mutation db,similarity:Belongs to the ATP-dependent DNA ligase family.,similarity:Contains 2 BRCT domains.,subunit:Binds to XRCC4. The LIG4-XRCC4 complex has probably a 1:2 stoichiometry. The LIG4-XRCC4 heteromer associates in a DNA-dependent manner with the DNA-dependent protein kinase complex DNA-PK, formed by the Ku p70/p86 dimer (G22P1/G22P2) and PRKDC.,tissue specificity:Testis, thymus, prostate and heart.,
  • 纯化
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存与保存

1.保存：-20℃，Avoid freeze/thaw cycles

2.有效期：1年

注意事项

1. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。 

2. 为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。 

3. 实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。 

 

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