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Vinculin (phospho Tyr821) Rabbit Polyclonal Antibody  Cite:0    分享

货号： IM51916
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  • 种属：H，M，R
  • 用途：WB，ELISA，IHC-P，IF-P，IF-F，IF-ICC
规格:
50μl 100μl
单位:
支
单价:￥1600.00
产品简介
储存与保存
注意事项
产品简介
  • 产品名称
    Vinculin (phospho Tyr821) Rabbit Polyclonal Antibody
  • 别名
    VCL; Vinculin; Metavinculin
  • 类别
    抗体产品
  • 基因名称
    VCL
  • 蛋白名称
    Vinculin
  • Clonality
    Polyclonal
  • 推荐应用
    WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA
  • 稀释度
    WB 1:500-2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:20000.Not yet tested in other applications.
  • 反应种属
    Human,Mouse,Rat
  • 浓度
    1 mg/ml
  • 存储缓冲液
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.
  • Human Gene ID
    7414
  • Human Swissprot No.
    P18206
  • Mouse Gene ID
    22330
  • Mouse Swissprot No.
    Q64727
  • Rat Gene ID
    305679
  • Rat Swissprot No.
    P85972
  • 免疫原
    The antiserum was produced against synthesized peptide derived from human Vinculin around the phosphorylation site of Tyr821. AA range:786-835
  • 特异性
    Phospho-Vinculin (Y821) Polyclonal Antibody detects endogenous levels of Vinculin protein only when phosphorylated at Y821.
  • 参考分子量
    124kDa
  • 宿主
    Rabbit
  • 同种型
    Rabbit,IgG
  • 背景介绍
    Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008],
  • 组织表达
    Metavinculin is muscle-specific.
  • 细胞定位
    Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, adherens junction . Cell junction, focal adhesion . Cytoplasm, cytoskeleton . Cell membrane, sarcolemma ; Peripheral membrane protein ; Cytoplasmic side . Recruitment to cell-cell junctions occurs in a myosin II-dependent manner. Interaction with CTNNB1 is necessary for its localization to the cell-cell junctions. .
  • 信号通路
    Focal adhesion;Adherens_Junction;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton
  • 功能
    disease:Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) [MIM:611407]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.,function:Involved in cell adhesion. May be involved in the attachment of the actin-based microfilaments to the plasma membrane. May also play important roles in cell morphology and locomotion.,online information:Vinculin entry,PTM:Aceylated; mainly by myristic acid but also small amount of palmitic acid.,PTM:Phosphorylated; on serines, threonines and tyrosines. Phosphorylation on Tyr-1133 in activated platelets affects head-tail interactions and cell spreading but has no effect on actin binding nor on localization to focal adhesion plaques.,similarity:Belongs to the vinculin/alpha-catenin family.,subcellular location:Cytoplasmic face of adhesion plaques.,subunit:Exhibits self-association properties. Interacts with NRAP and SORBS1 (By similarity). Interacts with TLN1. Interacts with SYNM.,tissue specificity:Metavinculin is muscle-specific.
  • 纯化
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
储存与保存

1.保存：-20℃，Avoid freeze/thaw cycles

2.有效期：1年

注意事项

1. 本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。 

2. 为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。 

3. 实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。 

 

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