产品名称

SRC-1 (Phospho Thr1179) Rabbit Polyclonal Antibody

别名

Nuclear receptor coactivator 1 (NCoA-1;EC 2.3.1.48;Class E basic helix-loop-helix protein 74;bHLHe74;Protein Hin-2;RIP160;Renal carcinoma antigen NY-REN-52;Steroid receptor coactivator 1;SRC-1);BHLHE74;SRC1

类别

抗体产品

基因名称

NCOA1

蛋白名称

SRC-1 (Phospho Thr1179)

Clonality

Polyclonal

推荐应用

WB,ELISA

稀释度

WB 1:1000-2000, ELISA 1:5000-20000

反应种属

Human,Mouse

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

8648

Human Swissprot No.

Q15788

Mouse Gene ID

17977

Mouse Swissprot No.

P70365

免疫原

Synthesized peptide derived from human SRC-1 (Phospho Thr1179)

特异性

This antibody detects endogenous levels of Human,Mouse SRC-1 (Phospho Thr1179)

预测分子量

158kDa

宿主

Rabbit

同种型

Rabbit,IgG

背景介绍

The protein encoded by this gene acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of this gene binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008],

组织表达

Widely expressed.

细胞定位

Nucleus

功能

catalytic activity:Acetyl-CoA + histone = CoA + acetylhistone.,disease:A chromosomal aberration involving NCOA1 is a cause of rhabdomyosarcoma. Translocation t(2;2)(q35;p23) with PAX3 generates the NCOA1-PAX3 oncogene consisting of the N-terminus part of PAX3 and the C-terminus part of NCOA1. The fusion protein acts as a transcriptional activator. Rhabdomyosarcoma is the most common soft tissue carcinoma in childhood, representing 5-8% of all malignancies in children.,domain:Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors. LXXLL motif 7, which is not present in isoform 2, increases the affinity for steroid receptors in vitro.,domain:The C-terminal (1107-1441) part mediates the histone acetyltransferase (HAT) activity.,function:Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response. Isoform 2 has a higher thyroid hormone-dependent transactivation activity than isoform 1 and isoform 3.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein.,PTM:Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites.,similarity:Belongs to the SRC/p160 nuclear receptor coactivator family.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,similarity:Contains 1 PAS (PER-ARNT-SIM) domain.,subunit:Interacts with the methyltransferase CARM1 (By similarity). Interacts with NCOA6 and NCOA2. Interacts with the FDL motif of STAT5A and STAT5B. Interacts with the LXXLL motif of STAT6. Interacts with STAT3 following IL-6 stimulation. Interacts with the basal transcription factor GTF2B. Interacts with the histone acetyltransferases EP300 and CREBBP. Interacts with PCAF, COPS5, NR3C1 and TTLL5/STAMP.,tissue specificity:Widely expressed.,

纯化

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.