产品名称

Chk1 Rabbit Polyclonal Antibody

别名

CHEK1; CHK1; Serine/threonine-protein kinase Chk1; CHK1 checkpoint homolog; Cell cycle checkpoint kinase; Checkpoint kinase-1

类别

抗体产品

基因名称

CHEK1

蛋白名称

Serine/threonine-protein kinase Chk1

Clonality

Polyclonal

推荐应用

WB,IHC-P,IF-P,IF-F,IF-ICC,ELISA

稀释度

WB 1:500-1:2000, IHC-P 1:100-1:300, IF-P/IF-F/IF-ICC 1:200-1:1000, ELISA 1:10000.Not yet tested in other applications.

反应种属

Human,Mouse,Rat

浓度

1 mg/ml

存储缓冲液

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% New type preservative N.

Human Gene ID

1111

Human Swissprot No.

O14757

Human Swissprot No.

12649

Mouse Swissprot No.

O35280

Rat Gene ID

140583

Rat Swissprot No.

Q91ZN7

免疫原

The antiserum was produced against synthesized peptide derived from human Chk1. AA range:286-335

特异性

Chk1 Polyclonal Antibody detects endogenous levels of Chk1 protein.

预测分子量

54kDa

宿主

Rabbit

同种型

Rabbit,IgG

背景介绍

The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2011],

组织表达

Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.

细胞定位

Nucleus . Chromosome . Cytoplasm . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Nuclear export is mediated at least in part by XPO1/CRM1 (PubMed:12676962). Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B (PubMed:15311285). Proteolytic cleavage at the C-terminus by SPRTN promotes removal from chromatin (PubMed:31316063). .

信号通路

Cell_Cycle_G1S;Cell_Cycle_G2M_DNA;p53;

功能

catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The autoinhibitory region (AIR) inhibits the activity of the kinase domain.,function:Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.,PTM:Phosphorylated by ATR in a RAD17-dependent manner in response to ultraviolet irradiation and inhibition of DNA replication. Phosphorylated by ATM in response to ionizing irradiation. ATM and ATR can both phosphorylate Ser-317 and Ser-345 and this results in enhanced kinase activity. Phosphorylation at Ser-345 also increases binding to 14-3-3 proteins and promotes nuclear retention. Conversely, dephosphorylation at Ser-345 by PPM1D may contribute to exit from checkpoint mediated cell cycle arrest. May also be phosphorylated at Ser-280 by AKT1/PKB, which may promote mono and/or diubiquitination. Also phosphorylated at undefined residues during mitotic arrest, which results in decreased activity.,PTM:Ubiquitinated. Mono or diubiquitination promotes nuclear exclusion.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.,similarity:Contains 1 protein kinase domain.,subcellular location:Nuclear export is mediated at least in part by XPO1/CRM1. Also localizes to the centrosome specifically during interphase, where it may protect centrosomal CDC2 kinase from inappropriate activation by cytoplasmic CDC25B.,subunit:Interacts with BRCA1, CLSPN, PPM1D, RAD51, TIMELESS, XPO1/CRM1 and YWHAZ/14-3-3 zeta.,tissue specificity:Expressed ubiquitously with the most abundant expression in thymus, testis, small intestine and colon.,

纯化

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.