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ROCK1 (12H12) Rabbit Monoclonal Antibody  Cite:0    分享

货号： IM68843
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  • 种属：H，M，R
  • 用途：WB，ELISA，IP，IHC-P，IF-P，IF-F，IF-ICC
规格:
50μl 100μl
单位:
支
单价:￥1300.00
产品简介
储存与保存
注意事项
产品简介
  • 产品货号
    IM68843
  • 别名
    ROCK1;Rho-associated protein kinase 1;Renal carcinoma antigen NY-REN-35;Rho-associated; coiled-coil-containing protein kinase 1;Rho-associated, coiled-coil-containing protein kinase I;ROCK-I;p160 ROCK-1;p160ROCK
  • 产品名称
    ROCK1 (12H12) Rabbit Monoclonal Antibody
  • 类别
    抗体产品
  • 蛋白名称
    ROCK1
  • Clonality
    Monoclonal
  • 推荐应用
    WB,IHC-P,IF-P,IF-F,IF-ICC,IP,ELISA
  • 反应种属
    Human,Mouse,Rat
  • 存储缓冲液
    PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
  • Human Gene ID
    6093
  • Human Swissprot No.
    Q13464
  • Mouse Gene ID
    19877
  • Mouse Swissprot No.
    P70335
  • Rat Gene ID
    81762
  • Rat Swissprot No.
    Q63644
  • 稀释度
    IHC-P 1:200-1:1000,WB 1:1000-1:5000,IF-P/IF-F/IF-ICC 1:200-1:1000,ELISA 1:5000-1:20000,IP 1:50-1:200
  • 参考分子量
    158kDa
  • 预测分子量
    158kDa
  • 序列
    Rho-associated protein kinase 1
  • 宿主
    Rabbit
  • 同种型
    Monoclonal, rabbit, IgG, Kappa
  • 背景介绍
    This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. [provided by RefSeq, Aug 2015]
  • 信号通路
    Chemokine;Vascular smooth muscle contraction;WNT;WNT-T CELLTGF-beta;Axon guidance;Focal adhesion;Leukocyte transendothelial migration;Regulates Actin and Cytoskeleton;Pathogenic Escherichia coli infection.
  • 功能
    catalytic activity:ATP + a protein = ADP + a phosphoprotein.,domain:The C-terminal auto-inhibitory domain interferes with kinase activity. RHOA binding leads to a conformation change and activation of the kinase. Truncated ROCK1 is constitutively activated.,enzyme regulation:Activated by RHOA binding.,function:Protein kinase that phosphorylates a large number of important signaling proteins, and thereby regulates the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Necessary for apoptotic membrane blebbing. Plays a role in smooth muscle contraction. Required for centromere positioning and centromere-dependent exit from mitosis.,miscellaneous:Inhibited by Y-27632.,PTM:Autophosphorylated on serine and threonine residues. Phosphorylated upon DNA damage, probably by ATM or ATR.,PTM:Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 PH domain.,similarity:Contains 1 phorbol-ester/DAG-type zinc finger.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 REM (Hr1) repeat.,subcellular location:Associated with the mother centriole and an intercentriolar linker (By similarity). A small proportion is associated with Golgi membranes.,subunit:Binds RHOA (activated by GTP). Interacts with ADD1, GEM, RHOB, RHOC, MYLC2B and VIM (By similarity). Binds RHOE, PPP1R12A, LIMK1 and LIMK2. Interacts with TSG101.,tissue specificity:Detected in blood platelets
  • 纯化
    Protein A
储存与保存

1.保存：-20℃

2.有效期：1年

注意事项

1.我司生产的生化试剂如无特殊标注，基本为非无菌包装，若用于细胞实验，请提前做好预处理。需低温保存的产品，一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。

2.本产品仅供科研使用。请勿用于医药、临床诊断或治疗，食品及化妆品等用途。请勿存放于普通住宅区。 

3.为了您的安全和健康，请穿好实验服并佩戴一次性手套和口罩操作。 

4.实验结果可由多种因素影响，相关处理只限于产品本身，不涉及其他赔偿。 

 

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